Cancer  .  Next-Generation Tools  .  Protein Stability

Selected Publications 

Fischer, T., Hartmann, O., Reissland, M., Prieto-Garcia, C., Klann, K., Pahor, N., Schulein-Volk, C., Baluapuri, A., Polat, B., Abazari, A., Gerhard-Hartmann, E., Kopp, H. G., Essmann, F., Rosenfeldt, M., Munch, C., Flentje, M. & Diefenbacher, M. E. (2022) PTEN mutant non-small cell lung cancer require ATM to suppress pro-apoptotic signalling and evade radiotherapy, Cell Biosci. 12, 50.                                                                                           

Prieto-Garcia, C., Hartmann, O., Reissland, M., Braun, F., Bozkurt, S., Pahor, N., Fuss, C., Schirbel, A., Schulein-Volk, C., Buchberger, A., Calzado Canale, M. A., Rosenfeldt, M., Dikic, I., Munch, C. & Diefenbacher, M. E. (2022) USP28 enables oncogenic transformation of respiratory cells, and its inhibition potentiates molecular therapy targeting mutant EGFR, BRAF and PI3K, Mol Oncol.                                                                                            

Mainz, L., Sarhan, M. A. F. E., Roth, S., Sauer, U., Maurus, K., Hartmann, E. M., Seibert, H.-D., Rosenwald, A., Diefenbacher, M. E. & Rosenfeldt, M. T. (2022) Autophagy Blockage Reduces the Incidence of Pancreatic Ductal Adenocarcinoma in the Context of Mutant Trp53, Frontiers in Cell and Developmental Biology. 10.

Mainz, L., Sarhan, M., Roth, S., Sauer, U., Kalogirou, C., Eckstein, M., Gerhard-Hartmann, E., Seibert, H. D., Voelker, H. U., Geppert, C., Rosenwald, A., Eilers, M., Schulze, A., Diefenbacher, M. & Rosenfeldt, M. T. (2022) Acute systemic knockdown of Atg7 is lethal and causes pancreatic destruction in shRNA transgenic mice, Autophagy, 1-14.

Ruiz, E. J., Lan, L., Diefenbacher, M. E., Riising, E. M., Da Costa, C., Chakraborty, A., Hoeck, J. D., Spencer-Dene, B., Kelly, G., David, J. P., Nye, E., Downward, J. & Behrens, A. (2021) JunD, not c-Jun, is the AP-1 transcription factor required for Ras-induced lung cancer, JCI Insight. 6.
Prieto-Garcia, C., Tomašković, I., Shah, V. J., Dikic, I. & Diefenbacher, M. (2021) USP28: Oncogene or Tumor Suppressor? A Unifying Paradigm for Squamous Cell Carcinoma, Cells. 10, 2652.

Prieto-Garcia, C., Hartmann, O., Reissland, M., Fischer, T., Maier, C. R., Rosenfeldt, M., Schulein-Volk, C., Klann, K., Kalb, R., Dikic, I., Munch, C. & Diefenbacher, M. E. (2021) Inhibition of USP28 overcomes Cisplatin-resistance of squamous tumors by suppression of the Fanconi anemia pathway, Cell Death Differ.

Meir, M., Kannapin, F., Diefenbacher, M., Ghoreishi, Y., Kollmann, C., Flemming, S., Germer, C. T., Waschke, J., Leven, P., Schneider, R., Wehner, S., Burkard, N. & Schlegel, N. (2021) Intestinal Epithelial Barrier Maturation by Enteric Glial Cells Is GDNF-Dependent, Int J Mol Sci. 22.

Hartmann, O., Reissland, M., Maier, C. R., Fischer, T., Prieto-Garcia, C., Baluapuri, A., Schwarz, J., Schmitz, W., Garrido-Rodriguez, M., Pahor, N., Davies, C. C., Bassermann, F., Orian, A., Wolf, E., Schulze, A., Calzado, M. A., Rosenfeldt, M. T. & Diefenbacher, M. E. (2021) Implementation of CRISPR/Cas9 Genome Editing to Generate Murine Lung Cancer Models That Depict the Mutational Landscape of Human Disease, Front Cell Dev Biol. 9, 641618.

Denk, S., Schmidt, S., Schurr, Y., Schwarz, G., Schote, F., Diefenbacher, M., Armendariz, C., Dejure, F., Eilers, M. & Wiegering, A. (2021) CIP2A regulates MYC translation (via its 5'UTR) in colorectal cancer, Int J Colorectal Dis. 36, 911-918.
Bauer, B., Liedtke, D., Jarzina, S., Stammler, E., Kreisel, K., Lalomia, V., Diefenbacher, M., Klopocki, E. & Mally, A. (2021) Exploration of zebrafish larvae as an alternative whole-animal model for nephrotoxicity testing, Toxicol Lett. 344, 69-81.

Prieto-Garcia, C., Hartmann, O., Reissland, M., Braun, F., Fischer, T., Walz, S., Schulein-Volk, C., Eilers, U., Ade, C. P., Calzado, M. A., Orian, A., Maric, H. M., Munch, C., Rosenfeldt, M., Eilers, M. & Diefenbacher, M. E. (2020) Maintaining protein stability of Np63 via USP28 is required by squamous cancer cells, EMBO Mol Med. 12, e11101.

Schmidt, S., Gay, D., Uthe, F. W., Denk, S., Paauwe, M., Matthes, N., Diefenbacher, M. E., Bryson, S., Warrander, F. C., Erhard, F., Ade, C. P., Baluapuri, A., Walz, S., Jackstadt, R., Ford, C., Vlachogiannis, G., Valeri, N., Otto, C., Schulein-Volk, C., Maurus, K., Schmitz, W., Knight, J. R. P., Wolf, E., Strathdee, D., Schulze, A., Germer, C. T., Rosenwald, A., Sansom, O. J., Eilers, M. & Wiegering, A. (2019) A MYC-GCN2-eIF2alpha negative feedback loop limits protein synthesis to prevent MYC-dependent apoptosis in colorectal cancer, Nat Cell Biol. 21, 1413-1424.

Ruiz, E. J., Diefenbacher, M. E., Nelson, J. K., Sancho, R., Pucci, F., Chakraborty, A., Moreno, P., Annibaldi, A., Liccardi, G., Encheva, V., Mitter, R., Rosenfeldt, M., Snijders, A. P., Meier, P., Calzado, M. A. & Behrens, A. (2019) LUBAC determines chemotherapy resistance in squamous cell lung cancer, J Exp Med. 216, 450-465.

Meir, M., Burkard, N., Ungewiss, H., Diefenbacher, M., Flemming, S., Kannapin, F., Germer, C. T., Schweinlin, M., Metzger, M., Waschke, J. & Schlegel, N. (2019) Neurotrophic factor GDNF regulates intestinal barrier function in inflammatory bowel disease, J Clin Invest. 129, 2824-2840.

Ungewiss, H., Rotzer, V., Meir, M., Fey, C., Diefenbacher, M., Schlegel, N. & Waschke, J. (2018) Dsg2 via Src-mediated transactivation shapes EGFR signaling towards cell adhesion, Cell Mol Life Sci. 75, 4251-4268.

Diefenbacher, M. & Orian, A. (2017) Stabilization of nuclear oncoproteins by RNF4 and the ubiquitin system in cancer, Mol Cell Oncol. 4, e1260671.

Cremona, C. A., Sancho, R., Diefenbacher, M. E., & Behrens, A. (2016). Fbw7 and its counteracting forces in stem cells and cancer: Oncoproteins in the balance. Semin Cancer Biol, 36, 52-61. doi: 10.1016/j.semcancer.2015.09.006

Diefenbacher, M.E., Chakraborty, A., Blake, S.M., Mitter, R., Popov, N., Eilers, M., and Behrens, A. (2015). Usp28 counteracts Fbw7 in intestinal homeostasis and cancer. Cancer research 75, 1181-1186.

Chakraborty, A., Diefenbacher, M.E., Mylona, A., Kassel, O., and Behrens, A. (2015). The E3 ubiquitin ligase Trim7 mediates c-Jun/AP-1 activation by Ras signalling. Nature communications 6, 6782.

Diefenbacher, M.E., Popov, N., Blake, S.M., Schulein-Volk, C., Nye, E., Spencer-Dene, B., Jaenicke, L.A., Eilers, M., and Behrens, A. (2014). The deubiquitinase USP28 controls intestinal homeostasis and promotes colorectal cancer. The Journal of clinical investigation 124, 3407-3418.

Schulein-Volk, C., Wolf, E., Zhu, J., Xu, W., Taranets, L., Hellmann, A., Janicke, L.A., Diefenbacher, M.E., Behrens, A., Eilers, M., et al. (2014). Dual regulation of Fbw7 function and oncogenic transformation by Usp28. Cell reports 9, 1099-1109.

Diefenbacher, M.E., Reich, D., Dahley, O., Kemler, D., Litfin, M., Herrlich, P., and Kassel, O. (2014). The LIM domain protein nTRIP6 recruits the mediator complex to AP-1-regulated promoters. PloS one 9, e97549.

Fritsch, R., de Krijger, I., Fritsch, K., George, R., Reason, B., Kumar, M.S., Diefenbacher, M., Stamp, G., and Downward, J. (2013). RAS and RHO families of GTPases directly regulate distinct phosphoinositide 3-kinase isoforms. Cell 153, 1050-1063.

Davies, C.C., Chakraborty, A., Diefenbacher, M.E., Skehel, M., and Behrens, A. (2013). Arginine methylation of the c-Jun coactivator RACO-1 is required for c-Jun/AP-1 activation. The EMBO journal 32, 1556-1567.

Castellano, E., Sheridan, C., Thin, M.Z., Nye, E., Spencer-Dene, B., Diefenbacher, M.E., Moore, C., Kumar, M.S., Murillo, M.M., Gronroos, E., et al. (2013). Requirement for interaction of PI3-kinase p110alpha with RAS in lung tumor maintenance. Cancer cell 24, 617-630.

Kumar, M.S., Hancock, D.C., Molina-Arcas, M., Steckel, M., East, P., Diefenbacher, M., Armenteros-Monterroso, E., Lassailly, F., Matthews, N., Nye, E., et al. (2012). The GATA2 transcriptional network is requisite for RAS oncogene-driven non-small cell lung cancer. Cell 149, 642-655.

Hauser, R., Pech, M., Kijek, J., Yamamoto, H., Titz, B., Naeve, F., Tovchigrechko, A., Yamamoto, K., Szaflarski, W., Takeuchi, N., Stellberger, T., Diefenbacher, M.E., Nierhaus, K.H., Uetz, P. (2012). RsfA (YbeB) proteins are conserved ribosomal silencing factors. PLoS genetics 8, e1002815.

Baumann, P., Thiele, W., Cremers, N., Muppala, S., Krachulec, J., Diefenbacher, M., Kassel, O., Mudduluru, G., Allgayer, H., Frame, M., et al. (2012). CD24 interacts with and promotes the activity of c-src within lipid rafts in breast cancer cells, thereby increasing integrin-dependent adhesion. Cellular and molecular life sciences : CMLS 69, 435-448.

Schreck, I., Al-Rawi, M., Mingot, J.M., Scholl, C., Diefenbacher, M.E., O'Donnell, P., Bohmann, D., and Weiss, C. (2011). c-Jun localizes to the nucleus independent of its phosphorylation by and interaction with JNK and vice versa promotes nuclear accumulation of JNK. Biochemical and biophysical research communications 407, 735-740.

Sancho, R., Jandke, A., Davis, H., Diefenbacher, M.E., Tomlinson, I., and Behrens, A. (2010). F-box and WD repeat domain-containing 7 regulates intestinal cell lineage commitment and is a haploinsufficient tumor suppressor. Gastroenterology 139, 929-941.

Roder, I.V., Choi, K.R., Reischl, M., Petersen, Y., Diefenbacher, M.E., Zaccolo, M., Pozzan, T., and Rudolf, R. (2010). Myosin Va cooperates with PKA RIalpha to mediate maintenance of the endplate in vivo. Proceedings of the National Academy of Sciences of the United States of America 107, 2031-2036.

Diefenbacher, M.E., Litfin, M., Herrlich, P., and Kassel, O. (2010). The nuclear isoform of the LIM domain protein Trip6 integrates activating and repressing signals at the promoter-bound glucocorticoid receptor. Molecular and cellular endocrinology 320, 58-66.

Diefenbacher, M., Sekula, S., Heilbock, C., Maier, J.V., Litfin, M., van Dam, H., Castellazzi, M., Herrlich, P., and Kassel, O. (2008). Restriction to Fos family members of Trip6-dependent coactivation and glucocorticoid receptor-dependent trans-repression of activator protein-1. Molecular endocrinology 22, 1767-1780.

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